Glucoamylasesareessentialenzymesthatcatalyzethehydrolysisofstarchintoglucose,playingacriticalroleinbothindustrialapplicationsandbiologicalprocesses.Understandingthecrystalstructureandevolutionarymechanismsofprokaryoticglucoamylasesprovidesvaluableinsightsintotheirfunctionaldiversityandcatalyticefficiency.Thisstudyfocusesonthestructuralcharacterizationandevolutionaryanalysisofaprokaryoticglucoamylase,revealingkeyfeaturessuchasdomainorganization,activesitearchitecture,andsubstrate-bindingmechanisms.Bycomparingitsstructurewithhomologousenzymes,wetracetheevolutionaryadaptationsthathaveoptimizeditsactivityandstabilityindiverseenvironments.Thesefindingsenhanceourknowledgeofglucoamylasefunctionandofferpotentialavenuesforenzymeengineeringtoimproveindustrialstarch-processingapplications.
